Analysis of the Flu SC2 Multiplex Assay Template

The CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay is a real-time reverse-transcriptase polymerase chain reaction (RT-PCR) test that detects and differentiates RNA from SARS-CoV-2, influenza A virus, and influenza B virus in upper or lower respiratory specimens. This video demonstrates how to set up the CDC Flu SC2 Multiplex Assay Template on an Applied Biosystems 7500 Fast Dx Real-time PCR Instrument using the optimal filter calibrations of FAM, Yakima Yellow, Texas Red X, and CY5. Learn more at www.cdc.gov/coronavirus/2019-ncov/lab/multiplex.html

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This video can also be viewed at
https://www.cdc.gov/coronavirus/2019-ncov/videos/labs/Multiplex-Assay-SetUp_lowres.mp4